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Flavonoid Biosynthesis


PUBLICATIONS

Zhang B and Schrader A (2017) TRANSPARENT TESTA GLABRA 1 – dependent regulation of the flavonoid biosynthesis. Plants 6(4).

Jaegle B, Uroic M K, Holtkotte X, Lucas C, Termath A O, Schmalz H-G, Bucher M, Hoecker U, Hulskamp M, Schrader A (2016) A fast and simple LC-MS-based characterization of the flavonoid biosynthesis pathway for few seed(ling)s. BMC plant biology, 16(1):190

Chopra D, Wolff H, Span J, Schellmann S, Coupland G, Albani M C, Schrader A & Hulskamp M (2014) Analysis of TTG1 function in Arabis alpina. BMC Plant Biol, 14, 16.

  

Light Signalling & Endoreduplication


 

PRODUCTION OF ANTHOCYANIN PIGMENT 2 - PAP2

Anthocyanidin accumulation is one of the COP1-dependent phenotypes. PAP2, a transcriptional activator of anthocyanidin biosynthesis, was found to interact with COP1. Competing for a target binding site in COP1, PAP2 was classified a putative target. Indeed, its stability is dependent on COP1.

PUBLICATIONS

Results from this project have been published with the Hoecker lab:

Maier A, Schrader A, Kokkelink L, Falke C, Welter B, Iniesto E, Rubio V, Uhrig JF, Hulskamp M, Hoecker U (2013) Light and the E3 ubiquitin ligase COP1/SPA control the protein stability of the MYB transcription factors PAP1 and PAP2 involved in anthocyanin accumulation in Arabidopsis. Plant J 74: 638-651

 

Topoisomerase VI - TOPOVI

TOPOVI components are required for normal levels of endoreduplication, chromatin condensation and silencing in A. thaliana. Results point to an activated ATM/ATR-dependent DNA damage repair and endoreduplication affecting checkpoint. (see work on MIDGET, one of the TOPOVI components)

TOPOVI mutants are defective in COP1-dependent phenotypes. Being identified as an interactor of COP1, we found that MIDGET links light signalling and endoreduplication. MIDGET does not show characteristics of a COP1 target – in contrast, it influences COP1 nuclear localization. Genetic interactions were found in respect to flowering time with COP1 and SPA1 mutants.

PUBLICATIONS

Schrader A, Uhrig J (2013) MIDGET cooperates with COP1 and SPA1 to repress flowering in Arabidopsis thaliana. Plant Signal Behav 8

Schrader A, Welter B, Hulskamp M, Hoecker U, Uhrig JF (2013) MIDGET connects COP1-dependent development with endoreduplication in Arabidopsis thaliana. Plant J 75: 67-79

Kirik V, Schrader A, Uhrig JF, Hulskamp M (2007) MIDGET unravels functions of the Arabidopsis topoisomerase VI complex in DNA endoreduplication, chromatin condensation, and transcriptional silencing. Plant Cell 19: 3100-3110

 

Quantitative Genetics


 

Filling the Toolbox: Method Development and Adaptation

PUBLICATIONS

Jaegle B, Uroic M K, Holtkotte X, Lucas C, Termath A O, Schmalz H-G, Bucher M, Hoecker U, Hulskamp M, Schrader A (2016) A fast and simple LC-MS-based characterization of the flavonoid biosynthesis pathway for few seed(ling)s. BMC plant biology, 16(1):190

Rishmawi L, Sun H, Schneeberger K, Hulskamp M, Schrader A (2014) Rapid identification of a natural knockout allele of ARMADILLO REPEAT-CONTAINING KINESIN 1 that causes root hair branching by mapping-by-sequencing. Plant physiology

Failmezger H, Jaegle B, Schrader A, Hulskamp M, Tresch A (2013) Semi-automated 3D leaf reconstruction and analysis of trichome patterning from light microscopic images. PLoS Comput Biol, 9(4):e1003029

 

GWAS

continuation of GWAS Projects

 

AraPop Project


“Our plant growth chamber has seen the world (see GWAS project) and now we are going local as well.”

In this project we analyze Cologne accessions under controlled conditions contributing to the AraPop project - a collaboration with the deMeaux and Linstädter group. See the AraPop website for more details.

 

Arabis alpina, Evolution & Epidermal Patterning Projects


 

Arabis alpina & Evolution Projects

Natural Variation and Evolution Group contributions:

Chopra D, Mapar M, Stephan L, Albani MC, Deneer A, Coupland G, Willing E-M, Schellmann S, Schneeberger K, Fleck C, Schrader A & Hülskamp M (2019) Genetic and molecular analysis of trichome development in Arabis alpina. Proceedings of the National Academy of Sciences

Zhang B, Chopra D, Schrader A & Huelskamp M (2019) Evolutionary comparison of competitive protein complex formation of MYB, bHLH and WDR proteins in plants. Journal of Experimental Botany

Chopra D, Wolff H, Span J, Schellmann S, Coupland G, Albani M C, Schrader A & Hulskamp M (2014) Analysis of TTG1 function in Arabis alpina. BMC Plant Biol, 14, 16.

 

Patterning Projects (A. thaliana)

Contributions to patterning projects:

Rishmawi L, Wolff H, Schrader A & Hulskamp M (2018) Sub-epidermal expression of ENHANCER OF TRIPTYCHON AND CAPRICE1 and its role in root hair formation upon Pi starvation. Front Plant Sci accepted (doi: 10.3389/fpls.2018.01411) 

Friede A, Zhang B, Herberth S, Pesch M, Schrader A & Hulskamp M (2017) The Second Intron Is Essential for the Transcriptional Control of the Arabidopsis thaliana GLABRA3 Gene in Leaves. Front Plant Sci, 8, 1382

Rishmawi L, Pesch M, Juengst C, Schauss A, Schrader A & Huelskamp M (2014) Non-cell-autonomous regulation of root hair patterning genes by WRKY75 in Arabidopsis thaliana. Plant Physiol.